The next generation of capillary electrophoresis instruments. The high resolution labchip is designed to be compatible with the glycan screening assay and low molecular weight protein assay on the labchip gxii microfluidic capillary electrophoresis cesds platform. The labchip gxii is an integral part of our analytical platform that allows us to quickly screen thousands of our protein expression strains for producing vaccines and protein drug candidates in a variety of therapeutic areas, said jeffrey allen, director of analytical biochemistry at pfenex. Assessment of protein product quality attributes and profiling of nglycans are performed in as few as forty seconds per sample. Aug 01, 2011 vaccine engineering, antibody quality screening, and glycosylation profiling processes can generate large quantities of protein samples that must be analyzed in a timely manner. Ensure that the chip, sample plate, ladder tube and buffer tube have been loaded properly on the labchip dx. Although still heavily relied upon, sodium dodecyle sulfate polyacrylamide gel electrophoresis sdspage is labor. A high throughput capillary electrophoresis method to obtain. The labchip gxii touch system is a complete solution for consistent and precise analysis of protein samples. A caliper labchip gxgxii instrument with labchip gxp software is a closed system 21 cfr 11.
Generation of highquality data and rapid, quantitative analyses are mandatory to determine next steps. Rapid analysis of nglycans on the labchip gxii microchipce. Antibody analysis using calipers labchip gxii system. Perkinelmer has led the way for over 25 years with powerful software and informatics solutions. Caliper life sciences functional and in good physical condition. Analysis of each sample is fast, occurring in 40 sec, and the samples are analyzed directly from a 96well plate. Labchip gx gxii and gx touch gxii touch instruments are for research use only and not for use in diagnostic procedures. Gene design, fusion technology and tev cleavage conditions.
Jan 17, 2017 all the quantitative values given in this manuscript are based on the calculation made by the labchip gxii software. Protein detection and quantification is required for the development of proteinbased therapeutics, as well as research into cell signaling, growth factors, and a. Sampling directly from a 96 or 384well plate, the lcgxii offers ease of use and walk away time. Mackenzie poskus lesley university greater boston area. In this method pngase freleased nglycans in a 96well microtiter plate are fluores. Caliper labchip gx automated electrophoresis rna dna analysis. Instrument safety the following safety information about the labchip gx touch gxii touch is included in this documentation. Seeplex labchip kit labchip dx user guide caliper page 8 of 24 pn 4594 rev 004 68 elm street hopkinton, ma 017481602 1877labchip 5222447 figure 6. Highresolution analysis provides exact sizing and quantitation of dna fragments and smears down to 5 bp from samples as small as 2 pgl. This separationsbased analytical technology platform consists of a variety of microfluidic chips. Leveraging microfluidics technology, the labchip gxii touch ht eliminates the.
Jul 16, 2008 the labchip gx represents a system targeted at genomics applications, while the gxii combines both genomics and protein research applications. Construction of phtpderivative vectors to express venom peptides in e. Labchip gxii touch protein characterization system perkinelmer. Gut microbes influence their hosts in many ways, in particular by modulating the impact of diet. Read about company and get contact details and address. Microfluidic electrophoresis analysis the labchip gx touch. Labchip gxii touch reducing time to result for protein characterization automated electrophoretic separations of rna, dna, proteins and glycans capillary electrophoresis performed in microfluidic chips sample directly from 96 or 384well microtiter plate comprehensive and quantitative analysis with labchip gx software. This labchip gx system has been inspected and tested by our technician to ensure. Increase efficiency and accelerate protein workflows with the. Protein and nucleic acids are separated via capillary isoelectric focusing cief and gel capillary electrophoresis, respectively, therefore requiring specialized ce systems. Leveraging microfluidics technology, the labchip gxii touch ht eliminates the need to handle time consuming sds page gels, and. Passed system diagnostics which included optics, plate handling, computer resources, chip interface, and test report genera. Caliper life sciences introduces labchip gx and gxii. George mantalas scientist downstream process development.
Rapid analysis of nglycans on the labchip gxii microchip. Our antivirus scan shows that this download is clean. Antibody analysis using calipers labchip gxii system abstract current initiatives in process analytical technology pat and quality by design qbd are driving the need to identify and thoroughly understand the relation between critical process parameters cpp and critical quality attributes cqas1. Furthermore, some ce systems, like agilents 7100 ce system, are comparable to liquid chromatography and are technically enhanced for analytical applications. Pe labchip gx touch ht dnarna characterization system. The main role involved improving visitor experience by offering excellent customer service, such as.
Labchip gxii touch microfluidics capillary electrophoresis. Labchip gxii touch biotherapeutics how a chip works. The caliper life sciences labchip cxii is a chipbased platform for analyzing dna, rna, protein, and glycan assays. Labchip gxgxii labchip technology advantages of labchip technology automated sampling from 96 and 384well plates transfer plates from a thermal cycler directly into the labchip gx system, no sample prep or transfer needed. Protein purification automated small scale purification. Labchip gxii touch ht protein characterization system perkinelmer. Right click on the link to the file and choose save target as and save the zip file to your desktop. The fold enrichment was determined from the sum of the mass of the heavy and light chains as measured by the labchip gxii data regression software. The instrument optics detect samples by laserinduced fluorescence. The labchip gxgxii systems offer researchers an automated alternative by streamlining the multiple, manual steps of slab gel electrophoresis, while also providing the throughput and data quality essential for life science laboratories today. Perkinelmer launches labchip gx touch and gxii touchtm. Labchip gx touchgx touch ii user manual perkinelmer. The labchip gx is designed for both rna and dna analysis, while the labchip gxii runs rna, dna, and also protein assays. In addition, as an alternative to ce, the labchip gx gxii delivers comparable data with as much as a 70x increase in throughput.
Used perkin elmer labchip gx touch 24 microfluidic. The labchip gxii is a complete solution for consistent and precise analysis of protein samples, and is an ideal instrument for protein research users. Automated, high performance electrophoresis for genomics manual steps of slab gel electrophoresis and labchip gx touch software is, view and download panasonic lumix dmcgx85 owners manual related manuals for panasonic since the departure date can be printed out using the software. It operates via standard gel electrophoresis principles with microvolumes for sample acquisition times as low as 40 seconds. Enrichment factors reported are calculated relative to the mouse igg2a standard run under identical conditions on the labchip gxii. See the complete profile on linkedin and discover mackenzies connections and jobs at similar companies. The labchip gxii touch protein characterization system provides the complete solution for reproducible quantitation, molecular weight sizing and percent purity analysis of protein samples. By leveraging microfluidic electrophoretic separation technology, it enables rapid characterization with minimal sample preparation setup and sample input volume. Use the links below to quickly find your software solution download. Perkinelmer labchip gx touch user manual pdf download. The principle of sds pagea full and clear explanation of the technique and how does it work duration.
It includes procedures for analyzing data from labchip gxgxii and gx touchgxii touch instruments, software troubleshooting, and using the optional labchip gxp software to provide compatibility with 21 cfr part 11 requirements. When powered on, the chip, eject, and status indicators labchip gxii light up. This caliper life sciences labchip gx ii automated electrophoresis system is. Contact a supplier or the parent company directly to get a quote or to find out a. A novel approach to automated highthroughput protein. There was a 4 log linear range of detection lower limit of detection was about 10 pg. D labchip gx touch gxii touch user manual perkinelmer, inc. Used labchip software to connect pc to labchip gx system.
Each individual sample can be analyzed in 41 seconds. The labchip gxii touch system quantifies protein attributes from process development through quality control in biotherapeutics development and manufacturing. Labchip gx touch nucleic acid analyzer perkinelmer. The labchip gx software displays the raw data as an electropherogram and generates a gellike image for visualization, data such as peak heights, peak areas, concentration, etc. Intuitive touch screen interface with comprehensive analysis using labchip gx software enabling true highthroughput analytics. Perkinelmer launches labchip gx touch and gxii touchtm automated gel electrophoresis systems. Any canceled orders or returns after the 14 days are subject to a 20% restocking fee. Labchip gx touch microfluidics capillary electrophoresis by. Biotherapeutics allow researchers to explore new mechanisms for the treatment of a variety of conditions. Labchip gx touch data analysis software allows users to visualize results via an electropherogram or virtual gel view. Buyers are responsible for all import fees, duties, and software license transfers, if applicable. After connecting a pc and opening the labchip software the status indicator turns green. The labchip gx touch gxii touch are classified as a class 1 device because the lasers are appropriately enclosed embedded and indicated with warning labels. Leveraging microfluidic electrophoretic separation technology, the labchip gxii touch system enables rapid characterization with minimal sample preparation setup and sample input volume.
Used caliper life sciences labchip gxii 2035202 dna. High resolution labchip gxii, touch ht perkinelmer. Labchip gxii separation is based on size, with the resolution ranging from 14 to 300 kda. Labchip gxii touch microfluidics capillary electrophoresis by perkinelmer, inc with sample acquisition time under 40 seconds, the labchip gxii touch can analyze 96 protein samples in less than an hour, virtually eliminating throughput bottlenecks.
Genomic dna kit labchip gx gxii user guide bioneer. The labchip gx and gxii provide high quality, accurate, and reproducible data that enable researchers to accelerate their discovery efforts, while saving time and money. The labchip gxgxii electrophoresis systems use a single sipper microfluidic chip to aspirate samples directly from 96 or 384well plates. The companys research portfolio includes a range of systems consisting of imaging, detection and extraction instrumentation for use on in vitro, ex vivo, and in vivo models, analysis hardware and software, plus a range of consumable. Labchip gxii touch ht protein characterization system. Preimplantation genetic testing perkinelmer applied genomics. The labchip gx series of instruments will be marketed by caliper and is designed to provide scientists novel benefits including extended walk away time, higher throughput and plate processing ability. Rapid analysis of nglycans on the labchip gxii microchipce platform abstract a high throughput microchipce method on the labchip gxii has been developed for profiling nglycans from recombinant igg antibodies. With complete analysis of genomic material in about 30 seconds, the labchip gx touch nucleic acid analyzer eliminates the nucleic acid quantitation workflow bottleneck. New automated platform provides life sciences researchers with precise nucleic acid quantification and protein characterization waltham, mass.
In this work, we used whole genome metagenomics to investigate the relationship between the gut metagenomes of dogs, humans, mice, and pigs. Caliper life sciences labchip gx ii automated electrophoresis. A high throughput capillary electrophoresis method to. It includes procedures for analyzing data from labchip gx gxii and gx touch gxii touch instruments, software troubleshooting, and using the optional labchip gxp software to provide compatibility with 21 cfr part 11 requirements.
The labchip gxii lcgxii platform shown in figure 1 provides an automated platform for rapid analysis of proteins and nucleic acids. This includes access security, data security and verification, and a complete set of audit logging. Product note rapid, costeffective oil condition monitoring spectrum oilexpress system. Less handson time reduces the manual labor associated with gels and frees up valuable resources. The labchip gxii is a complete solution for consistent and precise. May 26, 2016 the principle of sds pagea full and clear explanation of the technique and how does it work duration. The actual developer of the free software is perkinelmer inc. Labchip gxii touch biotherapeutics how a chip works youtube. Purchase orders, checks, and wire transfers are preferred.
These products, solutions and services support pharmaceutical and biotech companies, and academic institutions. Our biotherapeutics research products are designed for consistent and reproducible results, and are a component of glpcompliant processes in the worlds leading pharmaceutical, biotechnology and contract research organizations. Labchip gx touch microfluidics capillary electrophoresis. Perkinelmer india private limited offering perkinelmer labchip gxii touch ht capillary electrophoresis system in thane, maharashtra. The labchip platforms key feature is automation of conventional gel electrophoresis, eliminating the need for manual. View online or download perkinelmer labchip gxii touch user manual. Leveraging microfluidics technology, the labchip gxii touch ht eliminates the need to handle time consuming sds page gels, and provides superior results for less time and money. Principles of operation on page 20 usage labchip gx touch software is for use with labchip gx gxii and gx touch gxii touch instruments. Part 11 compatible software see data in real time or export for later analysis figure 1. Labchip gxii technology for vaccines and biologics calipers labonachip technology has become a critical tool in the research, development and manufacturing of vaccines and biotherapeutic drugs.
With sample acquisition time under 40 seconds, the labchip gxii touch can analyze 96 protein samples in less than an hour, virtually eliminating throughput bottlenecks and improving efficiency. Labchip gxii touch protein characterization system. Capillary electrophoresis system labchip gxii touch ht. The labchip gxii touch protein characterization system provides a complete solution for reproducible quantitation, molecular weight sizing and percent purity analysis of protein samples. Quick and accurate analysis of rna and dna samples the labchip gx is the most. Caliper labchip the labchip gxii is a complete solution for consistent and precise analysis of protein samples, and is an ideal instrument for protein research users. Jul 01, 2012 labchip gxii separation is based on size, with the resolution ranging from 14 to 300 kda. Software complies with fda 21cfr part 11 regulations. For smaller sample requirements, choose the gxii touch 24 for cost effective protein characterization up and down the workflow.
Whether it is quantitating monoclonal antibody titers, optimizing protein expression conditions, or rapidly screening igg nglycan profiles, the labchip gxii is the perfect. Labchip gx gxii with gx v3 and gxp security software. Both systems are compatible with 96 and 384 well plates, with the labchip gx having been designed as a low cost entry system targeting the needs of genomics researchers. After connecting a pc and opening the labchip software the status indicator. Blabchip gx touch gxii touch user manual perkinelmer, inc. The software contains an application security module for user account management and for access of controls to. The labchip gx represents a system targeted at genomics applications, while the gxii combines both genomics and protein research applications. The technology automatically stains distains, electrophoretically separates and analyzes the protein samples. Pe labchip gx touch ht dnarna characterization systemyear. Read perkinelmers applied genomics blog to learn how to improve the efficiency of your work in the applied genomics field. The system can be located in a designated laboratory room to limit system access to authorized individuals 21 cfr 11. The labchip gx touch and gxii touch benefit customers by reducing handson time and simplifying data generation via a user friendly operator touch screen.